Brain Perfusion MRI Findings in Patients with Behcet's Disease

Objective. To search brain perfusion MRI (pMRI) changes in Behcet's disease (BD) with or without neurological involvement. Materials and Method. The pMRI were performed in 34 patients with BD and 16 healthy controls. Based on neurologic examination and post-contrast MRI, 12 patients were classified as Neuro-Behcet (group 1, NBD) and 22 patients as BD without neurological involvement (group 2). Mean transit time (MTT), time to peak (TTP), relative cerebral blood volume (rCBV), and relative cerebral blood flow (rCBF) were obtained and compared to those of healthy control group (group 3). Results. There was a significant difference in the MTT and rCBF within the pons and parietal cortex in groups 1 and 2. rCBV increased in cerebral pedicle in group 1 compared with groups 2 and 3. In the temporal lobe white matter, prolonged MTT and decreased rCBF were found in groups 1 and 2. In the corpus striatum, internal capsule, and periventricular white matter, rCBF increased in group 1 compared with group 3 and decreased in groups 1 and 2. Conclusion. Brain pMRI is a very sensitive method to detect brain involvement in patients with BD and aids the clinical diagnosis of NBD, especially in patients with negative MRI findings

UTILIZATION OF IMMOBILIZED ENZYME IN DIAGNOSIS OF PHENYLKETONURIA

Bu çalısmada PAL enzimi poliester filmler üzerinde jelatine immobilize edilmistir. Serbest ve immobilize PAL enziminin aktiflik degerleri sırasıyla 2,6x10-2 ve 6,8x10-3 μmol/dk olarak bulunmustur. Serbest ve immobilize PAL enziminin spesifik aktivite degerleri ise sırasıyla 39,4x10-2 ve 13,9x10-2 μmol/dk.mg protein bulunmustur. Daha sonra immobilize PAL enzimi fenilketonüri teshisinde idrarda fenilalanin analizi için kullanılmıstır. İmmobilize PAL enzimi iki ay boyunca 17 kez kullanılmasına ragmen aktifligini korumustur. İmmobilize PAL enzimi idrardaki yüksek fenilalanin derisimlerinde daha az hatayla sonuç vermistir.In this study, PAL enzyme has been immobilized into gelatin on polyester films. Activity values of free and immobilized PAL enzyme has been found 2,6x10-2 and 6,8x10-3 μmol.min-1, respectively. Specific activity values of free and immobilized PAL enzyme has been found 39,4x10-2 and 13,9x10-2 μmol.min-1 .mg protein-1, respectively. Then immobilized PAL enzyme has been used for analysis of phenylalanine in urine for diagnosis of phenylketonuria. Although immobilized PAL enzyme has been used seventeen times for two months, it has kept its activity. Immobilized PAL enzyme has resulted with less error in high phenylalanine concentration in urine

Genetic polymorphisms variants in interleukin-6 and interleukin-1beta patients with obstructive sleep apnea syndrome in East Northern Turkey

Aim To investigate the relationship of IL-1β and IL-6 cytokine gene polymorphisms with obstructive sleep apnea syndrome (OSAS) in 61 patients admitted to the neurology clinic in Kafkas University Hospital with insomnia problem who were diagnosed with OSAS in sleeping labs, and 80 healthy subjects not associated with the syndrome. Methods Blood samples were taken to isolate DNA from patients diagnosed with OSAS based on polysomnography results and healthy controls. DNA amplification of the genes was performed with PCR. Amplification products were cut with the restriction enzymes in order to determine IL-1 gene (TaqI) and IL-6 gene (Lwel) polymorphisms. The cut DNA fragments were carried out in agarose gel electrophoresis, and RFLP analysis was performed by utilizing the images with gel imaging system. PCR products were sequenced with an Applied Biosystems Automated Sequencer. Results Polymorphic changes were observed for IL-1β gene in 26 of 62 patients (41.9%), and 16 of the 80 (25.8%) in the control group. The incidence of polymorphic changes in IL-6 gene was in seen in seven (of the 62 patients) (11.3%), and in the 16 (20%) controls. Conclusion The findings on the genomic level in OSAS may provide an important contribution to diagnosis of obstructive sleep apnea syndrome in clinical practice, as well as it helps to obtain the results easily about environmental and genetic interaction of OSAS patients

Chen Inequalities on Lightlike Hypersurface of a Lorentzian Manifold with Semi-Symmetric Metric Connection

WOS: 000412878500001In this paper, we introduce k-Ricci curvature and k-scalar curvature on lightlike hypersurface of a Lorentzian manifold with semi-symmetric metric connection. Using this curvatures, we establish some inequalities for lightlike hypersurface of a Lorentzian manifold with semi-symmetric metric connection. Considering these inequalities, we obtain the relation between Ricci curvature and scalar curvature endowed with semi-symmetric metric connection

The Frequency of Atopic Dermatitis and Other Skin Manifestations in Infants with Cow's Milk Protein Allergy in Karabuk, Turkey

Background Atopic dermatitis (AD) is the most common skin disease in childhood, and Cow's milk protein allergy (CMPA) is the most common cause of food allergies in infancy and may be characterized by atopic dermatitis with skin involvement as the first finding. The aim of this study is to determine the serum eosinophil cationic protein (sECP) levels, eosinophil counts and the frequency of AD and other skin manifestation among infants with CMPA. Materials and Methods: This cross-sectional study was conducted in Karabuk province, Turkey. Eighty-three infants who were diagnosed with CMPA and followed at the Karabuk Training Hospital Pediatric outpatient clinic. The first group consisted of 52 infants presenting with skin manifestations. The second group consisted of 31 infants who were not presenting skin manifestations. The sECP level of infants in both groups was measured using an Immulite 2000 XPi analyzer Immunoassay System (Germany). Results: Of 83 infants with CMPA, 62.6% (n=52) were detected skin involvement as the first finding. The proportions of atopic dermatitis and urticaria in CMPA infants with skin involvement were 90.4% and 9.6% respectively. The median sECP level and eosinophil counts (56.5 ng/mL vs 470/mm(3), p-value=0.001), in skin manifestations with CMPA group were significantly higher than that in the CMPA group without skin manifestations (33.1 ng/mL vs. 270/mm(3), p-value=0.006). Conclusion This study revealed that AD is the most common skin manifestation of CMPA and also found higher sECP levels in infants with skin involvement. The frequency of both diseases, which are easily treated with elimination diet, is increasing day by day.WOS:00063299050000

Comparison of the efficacy of low-level laser therapy and photodynamic therapy on oral mucositis in rats

Goncu, Beyza/0000-0001-6026-8218WOS: 000481755000023PubMed: 30826950Cancer treatment with chemotherapy or radiotherapy is associated with some side effects including in the oral cavity. One of the more significant oral complications is oral mucositis (OM) which induces severe pain and limits fundamental life behaviors such as eating, drinking, and talking. Although advancements in cancer treatment improved the survival rate, severe OM and opportunistic infection affect treatment adversely. Therefore, the control of OM is important for oral health quality of life and prognosis. Low-level laser therapy (LLLT) and photodynamic therapy (PT) are noninvasive methods that reduce inflammation and pain during wound healing. the aim of this study is to evaluate immunohistochemical and histological examination of the OM region of the PT comparing LLLT. in this study, 24 Sprague-Dawley rats were divided into three groups as control, LLLT, and PT groups. All groups received 5-fluorouracil intraperitoneally and a linear trauma to the mouth pouch with a needle. After the formation of OM in the mouth, the control group had no treatment; the LLLT group was administered LLLT, and the PT group had LLLT after indocyanine green application. Then all groups were sacrificed, and histological analyses and protein level detection of basic fibroblast growth factor (bFGF), transforming growth factor (TGF-beta), and platelet-derived growth factor (PDGF-BB) were evaluated in all groups. PT was determined to be more statistically significantly than LLLT with bFGF and PDGF-BB. However, regarding TGF-beta, no statistically significant difference was observed between the groups. Within the limitations of this study, indocyanine green may accelerate the LLLT effect. However, further studies on this subject are required.Bezmialem Vakif UniversityBezmialem Vakif University [9.2015/13]This study was funded by Bezmialem Vakif University (grant number 9.2015/13)

Penetration of moxifloxacin into rat mandibular bone and soft tissue

Objective. Based on its in vitro activity and spectrum of activity, the new 8-methoxyquinolone antibiotic moxifloxacin (MXF) seems suited for the antibiotic therapy of odontogenic infections. Penetration into the relevant tissue is another prerequisite for clinical efficacy. For this reason, the levels of MXF in plasma, soft tissue, and mandibular bone were determined in an animal model with Wistar rats. Material and methods. Samples of 49 rats were analyzed. Tissue samples were homogenized and proteins were precipitated. The pharmacokinetic evaluation was conducted based on non-compartmental analysis. Results. The concentration-time courses of tissues show a more plateau-shaped curve compared to plasma. Calculated AUC (area under the curve) ratios tissue:plasma were M. masseter:plasma = 2.64 and mandibles:plasma = 1.13. Conclusions. Administration of antibiotics is considered an important part of therapy during and/or after surgical procedures in the maxillofacial area. Because of the good penetration into bone and muscle tissues demonstrated in Wistar rats, MXF might be an option for clinical application in this indication